Statistical optimization of a novel excipient (CMEC) based gastro retentive floating tablets of propranolol HCl and it’s in vivo buoyancy characterization in healthy human volunteers
© Srikanth Meka et al.; licensee BioMed Central Ltd. 2012
Received: 6 July 2012
Accepted: 21 August 2012
Published: 30 August 2012
The objective of the present investigation is to formulate gastro retentive floating drug delivery systems (GRFDDS) of propranolol HCl by central composite design and to study the effect of formulation variables on floating lag time, D1hr (% drug release at 1 hr) and t90 (time required to release 90% of the drug). 3 factor central composite design was employed for the development of GRFDDS containing novel semi synthetic polymer carboxymethyl ethyl cellulose (CMEC) as a release retarding polymer. CMEC, sodium bicarbonate and Povidone concentrations were included as independent variables. The tablets were prepared by direct compression method and were evaluated for in vitro buoyancy and dissolution studies. From the polynomial model fitting statistical analysis, it was confirmed that the response floating lag time and D1hr is suggested to quadratic model and t90 is suggested to linear model. All the statistical formulations followed first order rate kinetics with non-Fickian diffusion mechanism. The desirability function was used to optimize the response variables, each having a different target, and the observed responses were highly agreed with experimental values. Statistically optimized formulation was characterized by FTIR and DSC studies and found no interactions between drug and polymer. The results demonstrate the feasibility of the model in the development of GRFDDS containing a propranolol HCl. Statistically optimized formulation was evaluated for in vivo buoyancy studies in healthy humans for both fed and fasted states. From the results, it was concluded that gastric residence time of the floating tablets were enhanced at fed stage but not in fasted state.
Drug delivery systems (DDS) are used for maximizing the therapeutic index of the drug and also targeted for reduction in the side effects. All over delivery systems the oral drug delivery has become the mainstay of treatment due to higher patient compliance and reduced patient discomfort. Under certain circumstances prolonging the gastric retention of a DDS is desirable for achieving greater therapeutic benefit of the drug . For example, drugs that are absorbed in the proximal part of the gastrointestinal tract (GIT), and the drugs that are less soluble or are degraded by the alkaline pH may benefit from prolong gastric retention. In addition, for local and sustained drug delivery to the stomach and the proximal small intestine to treat certain conditions, prolonging gastric retention of the therapeutic moiety may offer numerous advantages including improved bioavailability, therapeutic efficacy and possible reduction of the dose size [2–4]. All over the retentive systems gastric floating system for modulation of oral controlled drug delivery was found to be great importance. Hence in the present investigation effervescent floating systems were developed for prolonging the gastric retention.
In the present investigation propranolol HCl was selected as a model drug for the development of gastro retentive floating drug delivery systems (GRFDDS). Propranolol is a nonselective beta-adrenergic receptor blocking agent possessing no other autonomic nervous system activity used for the treatment of hypertension . It is highly lipophilic and almost completely absorbed after oral administration. However, it undergoes high first-pass metabolism by the liver and on average, only about 25% of propranolol reaches the systemic circulation . Variability of propranolol bioavailability is depends upon the secretory transporter P-glycoprotein (P-gp) located on the epithelium cells. Although P-gp appears to be distributed throughout the GIT, its levels are higher in more distal regions (stomach < jejunum < colon). Absorption through P-gp prolongs the drug exposure to CYP3A4. The colocalization of P-gp and CYP3A4 in the mature enterocytes and their overlapping substrate specificity reasonably suggests that the function of these two proteins may be synergistic and appear to be coordinately regulated. Consequently, a greater proportion of drug will be metabolized since the repetitive two-way kinetics (drug excerption from the enterocytes into the lumen via P-gp and reabsorption back into enterocytes) will simply prolong the drug exposure to CYP3A4. This mechanism not only limits the absorption of a wide variety of drugs, including peptides, but also poses a threat for potential drug interactions [7, 8].
Based on previously published literature, applications of gastro retentive drug delivery system (GRDDS) may be suitable for the drugs insoluble in intestinal fluids (acid soluble basic drugs), e.g., propranolol, metoprolol, diazepam . As discussed earlier, propranolol has short half-life, high first-pass metabolism, presence of food increases the bioavailability, P-gp plays important role in the absorption, and the drug is acid-soluble basic drug which make it suitable for GRDDS. A novel semi synthetic polymer carboxy methyl ethyl cellulose (CMEC) was used as release retarding polymer in the present investigation. Till now there were no reports found on CMEC as a release retarding polymer.
In the normal conventional optimization process, a single independent variable is varied while all others are kept constant at a specific set of conditions. It’s not possible to change more than one parameter at a time during the formulation development. This method may lead to unreliable results and improper conclusions besides wastage of excipients due to the requirement of large number of runs in achieving the desired goal. Response surface methodology (RSM) is an alternative to overcome this difficulty, which can be employed to optimize the formulations with suitable experimental design. RSM permits a deeper understanding of a process or product and has important applications like optimization and in establishing the robustness of that product. Central composite designs are a progression from the factorial designs which have been widely used in response-surface modeling and optimization .
The objective of the present investigation is to develop gastro retentive floating tablets (GRFT) of propranolol HCl using central composite design. In this study CMEC quantity, sodium bicarbonate concentration and Povidone concentration were selected as independent variables while floating lag time, D1hr and t90 were selected as dependent variables. For this study Design Expert software was used which gives information regarding critical values for achieving the desired response and also the possible interaction effects of selected independent variables on dependent variable.
Propranolol HCl was provided by Dr Reddy’s Laboratories Ltd (Hyderabad, India). CMEC, sodium bicarbonate, Povidone K 30 and magnesium stearate were obtained as gift samples from Unichem Laboratories Ltd (Goa, India). All other reagents and chemicals were of analytical grade.
Experimental range and levels of the independent variables in CMEC based formulations
Range and levels
CMEC (mg) X1
% w/w Sodium bicarbonate concentration X2
% w/w povidone concentration X3
X1 = CMEC quantity in mg
X2 = % w/w Sodium bicarbonate concentration (% w/w to the tablet weight)
X3 = % w/w Povidone concentration (% w/w to the tablet weight)
The response variables include
Y1 = Floating lag time (sec)
Y2 = D1hr (% drug released at 1 hr)
Y3 = t90 (time required to release 90% of the drug)
Preparation of GRFT of propranolol HCl
Formulations with the levels of independent variables and observed responses
CMEC quantity (mg) X1
%w/w of Sodium bicarbonate X2
%w/w of povidone X3
Floating lag time (sec)
Evaluation of GRFT
In vitro buoyancy studies
All the formulated floating tablets (n = 5) were subjected to in vitro buoyancy studies. The floating lag time was determined in one liter glass beaker containing 900 ml of 0.1 N HCl . The time required for the tablet to rise to the surface and float was determined as floating lag time. Results are given in Table 2.
In vitro dissolution studies
Zero order release kinetics
Where Qt is the amount of drug dissolved in time t, Q0 is the initial amount of drug in the solution (most times, Q0 = 0) and K0 is the zero order release constant expressed in units of concentration/time .
First order release kinetics
Where K is first order rate constant expressed in units of time-1.
Where C0 is the initial concentration of drug, k is the first order rate constant, and t is the time. The data obtained are plotted as log cumulative percentage of drug remaining vs. time which would yield a straight line with a slope of -K/2.303.
Where, k2 is the release rate constant.
A plot of the fraction of drug released against square root of time will be linear if the release obeys Higuchi equation. This equation describes drug release as a diffusion process based on the Fick’s law, square root time dependent .
Where, Mt is the amount of drug released at time t and M∝ is the amount released at time∝, thus the Mt/M∝ is the fraction of drug released at time t, k is the kinetic constant and n is the diffusion exponent.
In this model, the value of n characterizes the release mechanism of drug. For the case of cylindrical tablets, 0.45 = n corresponds to a Fickian diffusion mechanism, 0.45 < n < 0.89 to non-Fickian transport, n = 0.89 to Case II (relaxation) transport, and n > 0.89 to super case II transport .
Hixson - Crowell model
Where W0 is the initial amount of drug in the pharmaceutical dosage form, Wt is the remaining amount of drug in the pharmaceutical dosage form at time t and K (kappa) is a constant incorporating the surface – volume relation . The equation describes the release from systems where there is a change in surface area and diameter of particles or tablets.
Correlation coefficient values and release kinetics of GRFT
Statistical analysis of the data and optimization
Summary of ANOVA results in analyzing lack of fit (LOF) and pure error
Sum of squares
p value Prob > F
Floating lag time (Quadratic model)
Lack of Fit
D 1hr (Quadratic model)
Lack of Fit
t 90 (Linear model)
Lack of Fit
Floating lag time
Validation of the experimental design
Drug interaction studies
Fourier transformation-infrared spectroscopy (FTIR)
FTIR is used to identify the drug excipient interaction. FTIR studies were performed on drug, polymer and statistically optimized formulation. Samples were analyzed by potassium bromide pellet method in an IR spectrophotometer (Shimadzu, FTIR 8700) in the region between 3500-500 cm-1.
Differential scanning calorimetry (DSC)
Differential Scanning Calorimetric analysis of drug, polymer and statistically optimized formulation were done using Differential Scanning Calorimeter (Mettler Toledo Star SW 8.10, Model no: DSC 822). In this process about 8-10 mg of the samples were weighed in aluminum pan and were heated under nitrogen atmosphere from 5 °C to 250 °C.
In vivo buoyancy studies
To confirm the spatial and temporary placement of floating drug delivery system, a variety of techniques have been used like string technique, endoscopy and gamma scintigraphy [17–20]. Of these techniques, X-ray technique was used to determine the gastric residence time of the tablets. In the present investigation X-ray studies were conducted for the evaluation of intragastric floating behavior of the statistically optimized GRFT of propranolol HCl both in fasted and fed states.
The in vivo X-ray evaluation of floating ability studies were carried out by administering GRFT of propranolol HCl containing barium sulfate (BaSO4) in humans in fasted and fed state.
Two healthy male subjects of mean age 25 ± 2 yrs (ranging from 23 to 27), mean weight 68 ± 10 Kg (ranging from 58 to 78 kg) and a mean height of 170 ± 5 cm (ranging from 165 to 175 cm) participated in this study. The volunteers were judged healthy on the basis of their previous medical history, physical examination and routine laboratory tests. Both subjects were presented with full details of the investigation, verbally and in written form, prior to providing written informed consent and the study was conducted under the guidance of radiologist. The study was approved from an independent Institutional Ethics Committee of Andhra University, Visakhapatnam (India).
The statistically optimized GRFT of propranolol HCl was administered to the two volunteers, one under fasted and another one under fed states.
Fasted state: The subject was fasted overnight and then swallowed the gastric floating tablet with 200 ml of water. No food was allowed up to 3 hrs of dosing. Subject was not allowed to lay down for sleeping. Every one hour a glass of water (200 ml) was given.
Fed state: The subject was fasted overnight and in the morning given a high calorie-high fat breakfast with a total calorie value of approximately 900 Cal. The floating tablet was administered with 200 ml of water after half an hour of the breakfast. The subject was not allowed to eat anything up to 6 hrs but given a glass of water (200 ml) every hour.
Preparation of GRFT for in vivo studies
Optimized GRFT of propranolol HCl containing barium sulfate (PCMECRsoB) for in vivo X-ray evaluation were prepared by direct compression method. The amount of propranolol HCl was reduced to 40 mg for incorporating the barium sulfate (40 mg) as radio opaque substance to maintain the constant weight of the tablet. Propranolol HCl (40 mg) was geometrically mixed with CMEC until a homogeneous blend was achieved. Barium sulfate (40 mg), Povidone and sodium bicarbonate were added to the above blend, mixed and lubricated with magnesium stearate (1%w/w). Final blend was directly compressed into tablets on a 16-station rotary tablet punching machine (M/s. Cad mach Machinery Co Pvt Ltd. India) using 9 mm round plain punches at hardness of 4-6 kg/cm2.
Results and discussion
All the floating tablets were passed physicochemical tests like weight variation, assay and friability. Floating lag times of all the formulations were within the range of 280 to 720 sec (Table 2). As the concentration of sodium bicarbonate increases, the floating lag time found to be decreased.
The cumulative percent drug releases from GRFDDS prepared by central composite design with CMEC are shown in the Figure 1. From the results, it was observed that as the concentration of polymer increased along with concentration of sodium bicarbonate the drug release was retarded. This may be due to increased intensity of air pockets surrounding the jellified surface of the tablet. Increase in the concentration of the sodium bicarbonate at constant polymer concentration also retarded the drug release due to high intensity of the carbon dioxide gas pockets. Drug retardation was directly proportional to the concentration of Povidone which may be due to the formation of strong compactness between the particles .
All CMEC based formulations followed first order rate constant with non Fickian diffusion mechanism. (Table 3).
The responses of the floating tablets were fitted to linear, interaction and quadratic model using Design Expert software. As suggested by the software the responses floating lag time, and D1hr is suggested to quadratic model and t90 is suggested to linear model (Table 4).
By using semi synthetic polymer CMEC, 20 batches of formulations within the experimental design were prepared to obtain floating tablets which were evaluated for their floating lag time, D1hr and t90. From the ANOVA data, the F value for the floating lag time was found to be 1.12 which indicates that the model is non-significant, whereas for other responses D1hr and t90 the F value was found to be 16.04 and 21.28 respectively which indicates that both models are significant. The values of Prob > F less than 0.05 for all the responses except floating lag time are indicating that the models are significant. The response floating lag time exhibited Prob > F value 0.4269, which indicating model was not significant (Table 4). In the response observation for D1hr A, B, C, B^2 and C^2, for t90 A, B and C was found to be significant model terms. For floating lag time no significant model terms were found (A: CMEC, B: Sodium bicarbonate, C: Povidone). The lack of fit F value for floating lag time, D1hr and t90 was found to be 0.0111, 0.0003 and 0.0005 respectively implies that the lack of fit is significant. Similarly ‘R- squared’ value was also calculated for all responses. The ideal value is nearer to zero. ‘R- Squared’ value in the present model is near to zero which indicates towards a good model. In all the cases ‘Pred R squared’ values are in reasonable agreement with the ‘Adj R squared’ values except floating lag time (0.8769 & 0.5008 for D1hr and 0.7621 & 0.6484 for t90). A negative ‘Pred R squared’ was observed for the floating lag time response which implies that the overall mean is a better predictor of this response than current model. In all the case ‘Adeq Precision’ values are in the range of 12 – 17 except floating lag time which indicates an adequate signal and the model can be used to navigate the design space. For floating lag time ‘Adeq Precision’ was found to be 3.49 which indicates an inadequate signal and we should not used this model to navigate the design space (Table 5). Even though lack of fit was significant for all the variables, the model was preceded further because of the positive results obtained with other parameters such as F value, values of Prob > F, ‘R- squared’, Pred R squared’ and ‘Adj R squared’ for D1hr and t90 responses. Hence in the present investigation only D1hr and t90 responses were taken as dependent variables and optimization was proceeding with these parameters.
The contour and response surface plots for the all responses of all the formulation factors are shown in Figure 2-3. Contour and response plots of the response surface as a function of two factors at a time, holding all other factors at fixed levels, are more helpful in understanding both the main and the interaction effects of these two factors.
To optimize all the responses with different targets, a multi criteria decision approach like a numerical optimization technique by the desirability function and graphical optimization technique by the overlay plot were used (Figure 4). The optimized formulation was obtained by applying constraints on dependent variable responses and independent variables.
Optimized formulation was selected based on the criteria of less than 20% of the drug release at 1 hr (fixed by USP dissolution conditions ) and 90% of the drug released in between 10 to 11 hrs. Floating lag time was omitted in the optimization process as per the previous discussion. These constrains are common for all the formulations. The recommended concentrations of the independent variables were calculated by the Design Expert software from the above plots which has the highest desirability near to 1.0.
The optimum values of selected variables obtained by using Design Expert software was 248.88 mg of CMEC, 9.66% of sodium bicarbonate and 5.18% of Povidone for the development of GRFT of propranolol HCl.
Evaluation and validation of optimized formulations
The optimized formulation fulfilled all the criteria of physicochemical properties. In vitro buoyancy and dissolution studies were carried out on the prepared optimized formulations for verification of the theoretical prediction. Observed responses and predicted values for D1hr and t90 was found to be 17.21%, 10.3 hrs and 18.43%, 10 hrs respectively. The % relative error between the predicted values and experimental values of each response was calculated and the values were found to be 2.55% and 3.0% for D1hr and t90 respectively. From the results, it was concluded that these experimental findings are in close agreement with the model predictions which confirmed the predictability and validity of the model.
Drug interaction studies
Fourier transformation-infrared spectroscopy (FTIR)
The FTIR spectrum of CMEC showed the characteristic alcoholic –OH stretch at 3476 cm-1, C-H stretch at 2976 cm-1, -C = O stretch at 1761 cm-1 and -C-O-C asymmetric stretch at 1378 cm-1.
Statistically optimized CMEC based formulation (PCMECRso) showed all the characteristic peaks of propranolol HCl with minor shifts in its FTIR spectrum. This spectrum showed secondary amine –N–H stretch at 3280 cm-1, C-H stretch at 2974 cm-1, Aryl C = C stretch at 1579 cm-1, Aryl 0-CH2 asymmetric stretch at 1241 cm-1, Aryl 0-CH2 symmetric stretch at 1031 cm-1 and the peak at 797 cm-1 due to alpha- substituted naphthalene.
Differential scanning calorimetry
The absence of any changes in the FTIR spectra and DSC thermogram for the selected formulation indicated no chemical interaction between the CMEC and drug.
In vivo buoyancy studies
In vivo residence time of the optimized GRFT of propranolol HCl containing barium sulfate (PCMECRsoB)
Position of the tablet in GIT
Disappeared from gastric region
Disappeared from gastric region
Therefore, in the fed condition, the floating system showed a GRT prolonged by about 5 to 6 hrs over the fasted state.
The evaluation of the GRFT of propranolol HCl intragastric behavior in humans, showed the actual floatability of the tablet on the gastric content.
This study has demonstrated that in the fasted state under the influence of strong motor activity (the migrating myoelectric complex), there was no enhancement of GRT of gastro retentive floating tablet, where as there was a prolonged GRT of approximately 6 hrs in a fed state.
Thus the present study clearly indicated the applicability of the statistical optimization techniques for the prediction of the optimized concentrations of the excipients that influence the product parameters. These theoretical predictions can be verified for their experimental success as in the present case. The statistical optimization reduces the number of experiments to be carried for obtaining formulation with desired properties. Moreover the optimization is also useful in reducing the concentrations of the excipients to their optimum levels avoiding unnecessary wastage of excipients and thereby reducing the cost of the final product. The intragastric behavior of statistically optimized GRFT of propranolol HCl in humans, showed the floatability of the tablet on the gastric content. In vivo evaluation demonstrated no enhancement of GRT of gastro retentive floating tablet in fasted state, where as there was a prolonged GRT of approximately 6 hrs in the fed state. From the results, it is concluded that CMEC is novel semi synthetic polymer suitable for the development of GRFT of propranolol HCl.
The author is thankful to UGC (University Grants Commission, India) for awarding Senior Research Fellowship for carrying out this project. One of the authors, M.V.Srikanth, is thankful to K Praveen Kumar and C Vasu for providing valuable information to carry out the research work.
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