Pharmacological evaluation of the semi-purified fractions from the soft coral Eunicella singularis and isolation of pure compounds
© Deghrigue et al.; licensee BioMed Central Ltd. 2014
Received: 20 June 2014
Accepted: 4 September 2014
Published: 10 September 2014
Gorgonians of the genus Eunicella are known for possessing a wide range of pharmacological activities such as antiproliferative and antibacterial effect. The aim of this study was to evaluate the anti-inflammatory and gastroprotective effect of the organic extract and its semi-purified fractions from the white gorgonian Eunicella singularis and the isolation and identification of pure compound(s) from the more effective fraction.
Anti-inflammatory activity was evaluated, using the carrageenan-induced rat paw edema test and in comparison to the reference drug Acetylsalicylate of Lysine. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats. The purification of compound(s) from the more effective fraction was done by two chromatographic methods (HPLC and MPLC). The structure elucidation was determined by extensive spectroscopic analysis (1H and 13C NMR, COSY, HMBC, HMQC and NOESY) and by comparison with data reported in the literature.
The evaluation of the anti-inflammatory activity of different fractions from Eunicella singularis showed in a dependent dose manner an important anti-inflammatory activity of the ethanol fraction, the percentage of inhibition of edema, 3 h after carrageenan injection was 66.12%, more effective than the reference drug (56.32%). In addition, this ethanolic fraction showed an interesting gastroprotective effect compared to the reference drugs, ranitidine and omeprazol. The percentage of inhibition of gastric ulcer induced by HCl/ethanol in rats was 70.27%. The percentage of the reference drugs (ranitidine and omeprazol) were 65 and 87.53%, respectively. The purification and structure elucidation of compound(s) from this ethanolic fraction were leading to the isolation of five sterols: cholesterol (5α-cholest-5-en-3β-ol) (1); ergosterol (ergosta-5,22-dien-3β-ol) (2); stigmasterol (24-ethylcholesta-5,22-dien-3b-ol) (3); 5α,8α-epidioxyergosta 6,22-dien-3β-ol (4) and 3β-hydroxy-5α,8α-epidioxyergosta-6-ene (5); and one diterpenoid: palmonine D (6).
Based on data presented here, we concluded that diterpenoids and sterols detected in the ethanolic fraction can be responsible for its pharmacological activity.
KeywordsEunicella singularis Anti-inflammatory activity Gastroprotective effect Marine natural product Diterpenoid Sterols
Nature has developed an enormous diversity during several billion years of evolution. The Mediterranean Area represents one of the world’s major centers of animal diversity; with around 20 gorgonian species, four belong to the genus Eunicella: E. verrucosa, E. filiformis, E. cavolini and E. singularis.
Although natural compounds have been replaced by synthetic chemistry as the main source of new drugs, marine invertebrates remain an unequalled source of biochemical diversity. The studies on gorgonian have great importance in the research of marine resources of active compounds mainly by the pharmaceutical industry or for other uses. In fact, the gorgonians (Anthozoa, Gorgonacea) are known for possessing a wide range of pharmacologic and health promoting properties including antibacterial , antiviral , antiplasmodial , antifouling , antiproliferative , cytotoxic  and insecticidal  effects. The gorgonian of the genus Eunicella has been demonstrated to contain a wide variety of natural products as steroids and diterpenes ,. These compounds posses anticancer, gastroprotective and anti-inflammatory activities . For many years, our marine pharmacological group in Tunisia has been involved in an accurate research program on gorgonian constituents in order to define both their chemical composition and their biological activities. On the other hand, the use of non-steroidal anti-inflammatory drugs (NSAID) for the treatment of inflammatory diseases is associated with adverse effects as peptic ulcer . Therefore, the research of potent anti-inflammatory drugs from natural sources and with fewer side effects had become necessary. This study has yielded the anti-inflammatory and gastroprotective effects of the organic extract and its semi-purified fractions of the white gorgonian Eunicella singularis (Esper, 1791). The structure elucidation of the isolated compounds from the active fraction was done by 1D and 2D NMR experiments and by comparison with literature data.
HPLC was performed using a Waters model 510 pump equipped with Waters Rheodine injector and a differential refractometer, model 401. Medium pressure liquid chromatography (MPLC) was performed on a Buchi apparatus using a silica gel (230-400 mesh) column.
NMR spectra were obtained on Varian Inova 400 and Varian Inova 500 NMR spectrometers (1H at 400 and 500 MHz, 13C at 100 and 125 MHz, respectively) equipped with a Sun hardware, δ (ppm), J in hertz, and spectra referred to CD3Cl3 (δH=7.27; δC= 70.0 ) as internal standard. High-resolution ESIMS spectra were performed with a Micromass QTOF Micro mass spectrometer. All reagents were commercially obtained (Aldrich, Fluka) at the highest commercial quality and used without further purification except where noted. All reactions were monitored by TLC on silica gel plates (Macherey-Nagel). Carrageenan (BDH Chemicals Ltd Poole England), Acetylsalicylate of Lysine (ASL) were purchased from Sigma Chemical (Berlin, Germany). Ranitidine was obtained from Medis (Tunis, Tunisia), omeprazole was obtained from AstraZeneca (Monts).
Collection and extraction
E. singularis was collected from the Mediterranean Sea in various areas of the coastal region of Tabarka (Tunisia), in June 2010, at a depth between 20 and 30 m. Identification of specimens was carried out in the National Institute of Marine Sciences and Technologies (Salamboo, Tunisia) where a voucher specimen of E. singularis was deposited under the following reference 1132. After maceration of 600 g of the powdered material with methanol and dichloromethane (1:1, v/v) for 48h three times, the organic extract (40 g) was purified, using C18 cartridges (Sep-pack, Supelco), by gradient elution with different organic solvents in the order of decreased polarity: ethanol, acetone and methanol/CH2Cl2 (1:1) to give three semi-purified fractions: ethanol (F-EtOH), acetone (F-Ac) and methanol/CH2Cl2 (F-MeOH/CH2Cl2) fractions. Organic solvents were removed from recuperated fractions using rotating evaporator at 40°C.
Purification, isolation and structure elucidation
Fraction eluted with CH2Cl2:MeOH 95:5 (288 mg) was purified by HPLC on a Nucleodur 100-5 C18 (5 μ, 4.6 mm i.d. × 250 mm) with 95% MeOH:H2O as eluent (flow rate 1 mL/min) to give 0.1 mg of ergosta-5,22-dien-3β-ol (2) (tR=74 min) (Figure 1).
The CHCl3 extract (3.9 g) was chromatographed by silica gel MPLC using a solvent gradient system from CH2Cl2 to CH2Cl2: MeOH 1:1.
Fraction eluted with CH2Cl2: MeOH 95:5 (187.5 mg) was further purified by HPLC on a Nucleodur 100-5 C18 (5 μ, 4.6 mm i.d. × 250 mm) with 80% MeOH:H2O as eluent (flow rate 1 mL/min) to give 0.3 mg of 5α,8α-epidioxyergosta 6,22-dien-3β-ol (4) (tR=29 min), and 0.6 mg of 3β-hydroxy-5α,8α-epidioxyergosta-6-ene (5) (tR=2 min) and 3 mg of palmonine D (6) (tR=3 min) (Figure 1).
The purities of compounds were determined to be greater than 95% by HPLC and NMR. Furthermore, HPLC chromatograms and spectroscopic data of each compound were reported (Additional file 1).
Wistar rats of either sex, weighing 150-200 g were obtained from Pasteur Institute (Tunis, Tunisia). Housing conditions and in vivo experiments were approved according to the guidelines established by the European Union on Animal Care (CCE Council 86/609).
Carrageenan-Induced Rat Paw Edema
Gastric lesions induced by HCl/ethanol
The gastroprotective activity of the organic extract of E. singularis and its semi-purified fractions F-EtOH, F-Ac and F-MeOH/CH2Cl2 was studied in 150 mM HCl/EtOH induced gastric ulcer . Rats were divided into fifteen groups, fasted for 24 h prior receiving an intraperitoneal doses of vehicle (NaCl 9g/L, 2.5 mL/kg) for the control group, organic extract (50, 100 and 200 mg/kg, i.p.) and its semi-purified fractions F-EtOH, F-Ac and F-MeOH/CH2Cl2 (5, 10 and 25 mg/kg, i.p.) for the twelve test groups. Two other groups received ranitidine (60 mg/kg, i.p.) and omeprazole (30 mg/kg, i.p.) as reference drugs. After 30 min, all groups were orally treated with 1mL of 150 mM HCl/EtOH solution for gastric ulcer induction. Animals were killed 1 h after the administration of ulcerogenic agent, their stomachs were excised and opened along the great curvature, washed and stretched on cork plates. The surface was examined for the presence of lesions and the extent of the lesions was measured. The summative length of the lesions along the stomach was recorded (mm) as lesion index.
Data are presented as the mean±standard error (s.e.m). Statistical analysis was performed using Student’s t-test. The significance of difference was considered to include values of P<0.05.
Results and discussion
The current study was carried out to determine the in vivo anti-inflammatory and gastroprotective activities of the organic extract of E. singularis and its semi-purified fractions. The chemical composition of the more effective fraction was determined by both 1D and 2D NMR experiments.
Compound 1 was isolated as colorless powder. The molecular formula was determined to be C27H46O. Analysis of 1H and 13C NMR data evidenced a Δ5 dihydroxy-steroid structure with a saturated C8 cholestane side chain. Comparison with literature data allowed assigning the 5α-cholest-5-ene-3β-ol (cholesterol) structure .
Compound 2 has a molecular formula of C28H46O as determined by HRESIMS. Compound 2 was identified as ergosta-5,22-dien-3β-ol (ergosterol)  (Figure 2). This compound was also isolated from the soft coral Tubastraea coccinea and T. tagusensis.
Compound 3 was isolated as colorless powder. The molecular formula was determined to be C28H46O. Its identity was determined by 1D and 2D NMR data as 24-ethylcholesta-5,22-dien-3b-ol (stigmasterol) earlier isolated from the halophyte Salicornia herbacea and then reported also from the plant Aglaia eximia.
Compound 4 was isolated as white powder. The molecular formula was determined to be C28H44O3 by HRESIMS data. NMR data disclosed the 5α,8α-epidioxyergosta 6,22-dien-3β-ol structure. This compound was also isolated from the fungus Sporothrix schenckii and the fungus Cryptoporus volvatus.
Compound 5 has a molecular formula of C28H46O3 as determined by HRESIMS. 1H and 13C NMR indicated that compound 5 is the Δ22 derivative of compound 4. Therefore compound 5 was identified as 3β-hydroxy-5α,8α-epidioxyergosta-6-ene .
Compound 6 was isolated as colorless oil. The molecular formula was determined to be C24H36O6 by HRESIMS. The analysis of 1H NMR spectrum clearly revealed an eunicellin diterpenoid structure. The 13C NMR spectrum revealed 24 carbon signals. 1H- and 13CNMR assignments were carried out with the aid of the detailed 2D analyses (COSY, HMQC, NOESY, and HMBC) and the resulting NMR evidence revealed 6 to be defined as palmonine D . Three sterols named 5α, 8α-epidioxysterols, pregnanes and 9,11-secosterols were separated from E. cavolini, another specie of the genus Eunicella,. The five sterols identified in our study were isolated for the first time from E. singularis and were not yet found with another specie from this genus.
Palmonine D is also purified from E. labiata, another specie of the genus Eunicella. Other researchers reported the isolation of five diterpenoids from the gorgonian E. labiata, labiatamide A, labiatamide B, labiatin A, labiatin B and labiatin C  but these compounds were not yet separated from E. singularis. Massileunicellin A, was obtained from E. cavolini but also was not identified from E.singularis.
Anti-inflammatory effect of the intraperitoneal administration of E. singularis organic extract and its semi-purified fractions (F-EtOH, F-Ac and F-MeOH/CH 2 Cl 2 ) and of reference drug (Acetylsalicylate of Lysine; ASL) in carrageenan-induced rat paw edema test
Edema (10−2ml) (mean±s. e. m)
Edema inhibition (%)
Effect of E. singularis organic extract and its semi-purified fractions (F-EtOH, F-Ac, F-MeOH/CH 2 Cl 2 ), and of reference drugs (ranitidine and omeprazol) on gastric ulcer induced by HCl/ethanol in rats
Ulcer index (mm)
Furthermore, various phenolic compounds (alkaloids, glycosides, and saponins) detected in E. singularis organic extract and fractions  may be responsible for its activity. Several studies reported that alkaloids have anti-inflammatory and gastroprotective effects . Also, Glycosides, terpenoids and steroids detected in our samples are known to have anti-inflammatory and gastroprotective properties , The synergic effect of different compounds of E. singularis ethanolic fraction may be responsible for its higher anti-inflammatory and gastroprotective activities. Furthermore, the high free radical scavenging activity of F-EtOH in the DPPH test  suggests that the antioxidant activity may be one of the mechanisms of its gastroprotective and anti-inflammatory properties, because both ulcerous and inflammatory processes are related to an increase of free radicals .
In conclusion, the obtained results demonstrated that the ethanolic fraction of E. singularis had the highest activity in the two tests (anti-inflammatory and gastroprotective). The structure elucidation of compounds isolated from this fraction revealed the presence of five sterols and a eunicellan-based diterpenoid which may be responsible for its activity.
AB and HBJ were the supervisors and designed the study. MD carried out pharmacological activities. CF and LG contribute to the chemical study. SDM and MVDA carried out the structure elucidation. RBS made contribution to preparation of organic extract and fractions from E. singularis. All authors read and approved the final manuscript.
- Gori A, Bramanti L, Lopez-Gonzalez P, Thoma JN, Gili GM, Grinyo J, Uceira V, Rossi S: Characterization of the zooxanthellate and azooxanthellate morphotypes of the Mediterranean gorgonian Eunicella singularis. Mar Biol. 2012, 159: 1485-1496. 10.1007/s00227-012-1928-3.View ArticleGoogle Scholar
- McEnroe FJ, Fenical W: Structures and synthesis of some new antibacterial sesquiterpenoids from the gorgonian coral Pseudopterogorgia rigida. Tetrahedron. 1978, 34: 1661-1664. 10.1016/0040-4020(78)80198-7.View ArticleGoogle Scholar
- Groweiss A, Look S, Fenical W: Solenolides, new antiinflammatory and antiviral diterpenoids from a marine octocoral of the genus Solenopodium. J Org Chem. 1988, 53: 2401-2406. 10.1021/jo00246a001.View ArticleGoogle Scholar
- Wei X, Rodriguez AD, Baran P, Raptis RG, Sanchez JA, Ortega-Barria E, Gonzalez J: Antiplasmodial cembradiene diterpenoids from a Southwestern Caribbean gorgonian octocoral of the genus Eunicea. Tetrahedron. 2004, 60: 11813-11819. 10.1016/j.tet.2004.09.108.View ArticleGoogle Scholar
- Qi SH, Zhang S, Qian PY, Xiao ZH, Li MY: Ten new antifouling briarane diterpenoids from the South China Sea gorgonian Junceella juncea. Tetrahedron. 2006, 62: 9123-9130. 10.1016/j.tet.2006.07.049.View ArticleGoogle Scholar
- Deghrigue M, Dellai A, Bouraoui A: In vitro antiproliferative and antioxidant activities of the organic extract and its semi-purified fractions from the Mediterranean gorgonian Eunicella singularis. Int J Pharm Pharm Sci. 2013, 5: 432-439.Google Scholar
- Sheu JH, Sung PJ, Cheng MC, Liu HY, Fang LS, Duh CY, Chiang MY: Novel Cytotoxic Diterpenes, Excavatolides A-E, Isolated from the Formosan Gorgonian Briareum excavatum. J Nat Prod. 1998, 61: 602-608. 10.1021/np970553w.View ArticlePubMedGoogle Scholar
- Grode SH, James TR, Cardellina JH, Onan KD: Molecular structures of the briantheins, new insecticidal diterpenes from Briareum polyanthes. J Org Chem. 1983, 48: 5203-5207. 10.1021/jo00174a010.View ArticleGoogle Scholar
- Berrue F, Kerr RG: Diterpenes from gorgonian corals. Nat Prod Rep. 2009, 26: 681-710. 10.1039/b821918b.View ArticlePubMedGoogle Scholar
- Ioannou E, Abdel-Razik AF, Alexi X, Vagias C, Alexis MN, Roussis V: Pregnanes with antiproliferative activity from the gorgonian Eunicella cavolini. Tetrahedron. 2008, 64: 11797-11801. 10.1016/j.tet.2008.09.078.View ArticleGoogle Scholar
- Ioannou E, Abdel-Razik AF, Alexi X, Vagias C, Alexis MN, Roussis V: 9,11-Secosterols with antiproliferative activity from the gorgonian Eunicella cavolini. Bioorg Med Chem. 2009, 17: 4537-4541. 10.1016/j.bmc.2009.05.004.View ArticlePubMedGoogle Scholar
- Hossain H, Al-Mansur A, Akter S, Sara U, Ahmed MR, Jahangir AA: Evaluation of anti-inflammatory activity and total tannin content from the leaves of Bacopa monnieri (Linn.). IJPSR. 2014, 5 (4): 1246-1252.Google Scholar
- Kupchan SM, Britton RW, Ziegler MF, Sigel CW: Bruceantin, a new potent antileukemic simaroubolide from Brucea antidysenterica. J Org Chem. 1973, 38: 178-179. 10.1021/jo00941a049.View ArticlePubMedGoogle Scholar
- Winter CA, Risley EA, Nuss GW: Carrageenan induced edema hind paw of the rat as an easy for anti-inflammatory drugs. Proc Soc Exp Biol Med. 1962, 3: 544-547. 10.3181/00379727-111-27849.View ArticleGoogle Scholar
- Mizui T, Doteuchi M: Effect of polyamines on acidified ethanol-induced gastric lesions in rats. Japanese J Pharmacology. 1983, 33: 939-945. 10.1254/jjp.33.939.View ArticleGoogle Scholar
- Acimovic J, Rozman D: Steroidal triterpenes of cholesterol synthesis. Molecules. 2013, 18: 4002-4017. 10.3390/molecules18044002.View ArticlePubMedGoogle Scholar
- Lages BG, Fleury BG, Hovell AMC, Rezende CM, Pinto AC, Creed JC: Proximity to competitors changes secondary metabolites of non-indigenous cup corals, Tubastraea spp., in the southwest Atlantic. Mar Biol. 2012, 159: 1551-1559. 10.1007/s00227-012-1941-6.View ArticleGoogle Scholar
- Wang X, Zhang M, Zhao Y, Wang H, Liu T, Xin Z: Pentadecyl ferulate, a potent antioxidant and antiproliferative agent from the halophyte Salicornia herbacea. Food Chem. 2013, 141: 2066-2074. 10.1016/j.foodchem.2013.05.043.View ArticlePubMedGoogle Scholar
- Harneti D, Supriadin A, Ulfah M, Safari A, Supratman U, Awang K, Hayashi H: Cytotoxic constituents from the bark of Aglaia eximia (Meliaceae). Phytochem Lett. 2014, 8: 28-31. 10.1016/j.phytol.2014.01.005.View ArticleGoogle Scholar
- Sgarbi DBG, da Silva AJR, Carlos IZ, Silva CL, Angluster J, Alviano CS: Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii. Mycopathologia. 1997, 139: 9-14. 10.1023/A:1006803832164.View ArticlePubMedGoogle Scholar
- Wei-Guang M, Xing-Cong L, De-Zu W, Chong-Ren Y: Ergosterol peroxides from Cryptoporus volvatus. Acta Bot Yunnanica. 1994, 16 (2): 196-200.Google Scholar
- Ortega MJ, Zubia E, Salva J: A new cladiellane diterpenoid from Eunicella labiata. J Nat Prod. 1997, 60: 485-487. 10.1021/np970026c.View ArticleGoogle Scholar
- Roussis V, Fenical W, Vagias C, Kornprobst JM, Miralles J: Labiatamides A, B, and other eunicellan diterpenoids from the Senegalese gorgonian Eunicella labiata. Tetrahedron. 1996, 52: 2735-2742. 10.1016/0040-4020(96)00010-5.View ArticleGoogle Scholar
- Hanson JR: Diterpenoids. Nat Prod Rep. 2001, 18: 88-94. 10.1039/b007477m.View ArticlePubMedGoogle Scholar
- Borgi W, Ghedira K, Chouchane N: Antiinflammatory and analgesic activities of Zizyphus lotus root barks. Fitoterapia. 2007, 78: 16-19. 10.1016/j.fitote.2006.09.010.View ArticlePubMedGoogle Scholar
- Inoue H, Ohshima H, Kono H, Yamanaka M, Kubota T, Aihara M, Hiroi T, Yago N, Ishida H: Supressive effects of tranilast on the expression of inducible cyclooxygenase (COX-2) in interleukin-1-β-stimulated fibroblasts. Biochem Pharmacol. 1997, 53: 1941-1944. 10.1016/S0006-2952(97)00187-1.View ArticlePubMedGoogle Scholar
- Ishihara M, Ito M: Influence of aging on gastric ulcer healing activities of cimetidine and omeprazole. Eur J Pharmacol. 2002, 444: 209-215. 10.1016/S0014-2999(02)01651-5.View ArticlePubMedGoogle Scholar
- Schmeda-Hirschmann G, Astudillo L, Rodriguez J, Theoduloz C, Yanez T: Gastroprotective effect of the Mapuche crude drug Araucaria araucana resin and its main constituents. J Ethnopharmacol. 2005, 101: 271-276. 10.1016/j.jep.2005.04.027.View ArticlePubMedGoogle Scholar
- Farina C, Pinza M, Pifferi G: Synthesis and anti-ulcer activity of new derivatives of glycyrrhetic, oleanolic and ursolic acids. Il Farmaco. 1998, 53: 22-32. 10.1016/S0014-827X(97)00013-X.View ArticlePubMedGoogle Scholar
- Hiruma-Lima CA, Gracioso JS, Toma W, Paula ACB, Almeida ABA, Brasil DD, Muller AH, Souza-Brito AR: Evaluation of the gastroprotective activity of cordatin, a diterpene isolated from Aparisthmium cordatum (Euphorbiaceae). Biol Pharm Bull. 2000, 23: 1465-1469. 10.1248/bpb.23.1465.View ArticlePubMedGoogle Scholar
- Moulin M, Coquerel A: Pharmacologie, connaissance et pratique. 2002, Masson, ParisGoogle Scholar
- Radjasa OK, Vaske YM, Navarro G, Vervoort HC, Tenney K, Linington RG, Crews P: Highlights of marine invertebrate-derived biosynthetic products: their biomedical potential and possible production by microbial associants. Bioorg Med Chem. 2011, 19: 6658-6674. 10.1016/j.bmc.2011.07.017.PubMed CentralView ArticlePubMedGoogle Scholar
- Pedernera AM, Guardia T, Guardia Calderon C, Rotelli AE, de la Rocha NE, Di Genaro S, Pelzer LE: Anti-ulcerogenic and anti-inflammatory activity of the methanolic extract of Larrea divaricata Cav. In rats. J Ethnopharmacol. 2006, 105: 415-420. 10.1016/j.jep.2005.11.016.View ArticlePubMedGoogle Scholar
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